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Microscope field of view calculator

Microscope field of view, from the sensor (rectangular field) or from the eyepiece field number (circular field).

Parameters

px
px
Sensor pixel size
×
Note

Binning changes the projected pixel size and the number of pixels read out, not the field of view: the sensor area stays the same.

Result

Field of view

665.6 × 665.6 µm

Calculation
field = sensor / magnification = 13 312 × 13 312 µm / 20× = 665.6 × 665.6 µm
Physical sensor
13 312 × 13 312 µm
Pixels read out
2048 × 2048 px
Total optical magnification
20×
Field area
443 023 µm²

Calculation, assumptions and sources

OverviewWidth, height or diameter, and area of the field observed through a microscope.

Width, height or diameter, and area of the field observed through a microscope. The rectangular field recorded by the camera is computed from the sensor dimensions in pixels and the pixel size; the circular field seen through the eyepiece, from the field number.

Method & formulascamera field = sensor dimension in pixels × pixel size / total magnification
camera field = sensor dimension in pixels × pixel size / total magnificationeyepiece diameter = field number / total magnification

The first refers the sensor area, a rectangle, back to the specimen; the second, the eyepiece field diaphragm, a disc. The two fields differ: the camera records only a portion inscribed within the visual field.

Field number
diameter in millimetres of the field diaphragm, engraved on the eyepiece.
Effect of magnification
the field is inversely proportional to magnification. From 20× to 40×, each dimension halves and the area quarters.
Validity domainThe calculation assumes the sensor is fully illuminated.

The calculation assumes the sensor is fully illuminated. An adapter that is too narrow, or a sensor larger than the available image field, produces vignetting, which reduces the usable field. For a measurement, calibrate with a stage micrometer.