Biology
Biomedical and biological calculations.
- DNA primer melting temperature (Tm)Calculate a DNA primer's Tm with the nearest-neighbor thermodynamic model (SantaLucia, 1998), with the classic empirical formulas shown for comparison.
- DNA sequence analyzerAnalyze a DNA sequence: base composition, GC content, complement and reverse complement, with support for ambiguous IUPAC codes.
- DNA to protein translatorTranslate a DNA sequence into protein in any of the six reading frames (+1, +2, +3, −1, −2, −3): codons, amino acids in 1 or 3 letters, stop codons.
- ORF detector (open reading frames)Detect open reading frames (ORFs) in a DNA sequence across all six frames: position, length, protein, careful handling of ambiguous bases.
- PCR primer calculatorAnalyze and compare two PCR primers: length, GC content, Tm, GC clamp, homopolymers, complementarity, dimer and hairpin risk.