Biology
Molecular biology, genetics, microbiology and cell culture.
- DNA primer melting temperature (Tm)A DNA primer's Tm by the nearest-neighbor thermodynamic model (SantaLucia, 1998), with the empirical formulas for comparison.Provisional
- DNA sequence analyzerAnalysis of a DNA sequence: base composition, GC content, complement and reverse complement, with ambiguous IUPAC codes supported.
- DNA to protein translatorA DNA sequence translated into protein in any of the six frames (+1, +2, +3, −1, −2, −3): codons, amino acids in one- or three-letter code, stop codons.
- DNA/RNA concentration and purity (A260)Absorbance at 260 nm to a concentration (ng/µL), and purity read from the 260/280 and 260/230 ratios, with their thresholds.
- ORF detector (open reading frames)Open reading frames (ORFs) of a DNA sequence across all six frames: position, length, protein, ambiguous bases handled.
- PCR primer calculatorAnalysis and comparison of two PCR primers: length, GC content, Tm, GC clamp, homopolymers, complementarity, dimer and hairpin risk.
Genetics
3 tools
- Genetic drift simulator (Wright-Fisher)Simulated genetic drift: ten Wright-Fisher populations with a reproducible seed, heterozygosity H₀(1 − 1/2N)^g, fixed, lost or drifting tally.Provisional
- Hardy-Weinberg equilibriumAllele and genotype frequencies (p² + 2pq + q² = 1) from the recessive phenotype, the allele or observed counts.
- Punnett square calculatorThe square of a mono or dihybrid cross: genotype and phenotype ratios, from 3:1 to 9:3:3:1.
Microbiology & cell culture
3 tools
- Bacterial generation timeA culture’s generation time from two counts, population after a duration, or time to reach a target.
- Cell counting (counting chamber)A chamber count (Neubauer, Malassez) in cells/mL, with trypan blue viability and the Poisson uncertainty.
- CFU count calculator and decimal dilutionsFrom counted colonies to the concentration (CFU/mL), or the decimal dilution that makes a plate countable (30 to 300).