Molecular biology & biochemistry
Sequences, primers, proteins and biochemical calculations.
- DNA primer melting temperature (Tm)A DNA primer's Tm by the nearest-neighbor thermodynamic model (SantaLucia, 1998), with the empirical formulas for comparison.Provisional
- DNA sequence analyzerAnalysis of a DNA sequence: base composition, GC content, complement and reverse complement, with ambiguous IUPAC codes supported.
- DNA to protein translatorA DNA sequence translated into protein in any of the six frames (+1, +2, +3, −1, −2, −3): codons, amino acids in one- or three-letter code, stop codons.
- DNA/RNA concentration and purity (A260)Absorbance at 260 nm to a concentration (ng/µL), and purity read from the 260/280 and 260/230 ratios, with their thresholds.
- ORF detector (open reading frames)Open reading frames (ORFs) of a DNA sequence across all six frames: position, length, protein, ambiguous bases handled.
- PCR primer calculatorAnalysis and comparison of two PCR primers: length, GC content, Tm, GC clamp, homopolymers, complementarity, dimer and hairpin risk.